An Introduction to Fluorescence Correlation Spectroscopy
暫譯: 螢光相關光譜學入門
Thorsten Professor Wohland , Sudipta Professor Maiti , Radek Professor Machan
- 出版商: IOP Publishing Ltd
- 出版日期: 2020-11-04
- 售價: $6,400
- 貴賓價: 9.5 折 $6,080
- 語言: 英文
- 頁數: 300
- 裝訂: Hardcover - also called cloth, retail trade, or trade
- ISBN: 0750320788
- ISBN-13: 9780750320788
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相關主題
商品描述
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them.
This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting.
Key Features
- Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook.
- Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes.
- Provides rigorous mathematical derivations of fundamental FCS equations and models.
- Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.
商品描述(中文翻譯)
《螢光相關光譜學入門》是一本全面介紹螢光相關光譜學（FCS）的書籍，這是一種生物物理實驗技術，越來越多地用於研究和量化分子在體外以及在活細胞和多細胞生物中的流動性、濃度和相互作用。對於初學FCS的學生和研究人員來說，這本書可以作為該技術的首次介紹，而那些已經在研究中定期使用FCS的人則可能會發現這本書有助於加深對該技術、其可能性、限制和潛在陷阱的理解，以及避免這些陷阱的方法。
本書向讀者介紹了FCS在研究中實際使用所需的所有方面。首先解釋了螢光強度波動的概念及其自相關和交叉相關函數，以幫助讀者理解其基本原理。接下來是儀器FCS設置的概述以及各種數據收集和處理的方法，推導出將實驗獲得的相關函數與基礎分子過程相關的理論模型，並描述如何用這些模型擬合實驗數據。數學上更為複雜的部分與其餘文本分開，讀者可以輕鬆跳過這些部分，專注於FCS的概念和實際應用。本書包含互動圖形，並附有一個互動可計算文檔文件，允許讀者測試FCS結果對各種實驗參數的依賴性，並獲得FCS數據擬合的實際見解。
主要特點：
- 以易於理解的方式介紹FCS的概念，並在電子書中提供動畫和圖形支持。
- 包含一個補充的互動可計算文檔文件，允許讀者實驗各種FCS設置和擬合參數，幫助讀者測試他們的理解並模擬實驗結果。
- 提供基本FCS方程和模型的嚴謹數學推導。
- 教學特點包括問題、簡短回顧和對與特定章節相關文獻的批判性討論，涵蓋FCS領域的應用和基本發展。
作者簡介
Thorsten Wohland is a professor in the departments of Biological Sciences and Chemistry at the National University of Singapore (NUS). He was awarded an Alexander von Humboldt research fellowship and the Outstanding Scientist Award of the faculty of science at NUS. His interests lie in the development of fluorescence techniques and their application to biological systems. He continues to develop new FCS modalities and is passionate about teaching FCS, which motivated the writing of this book.
Sudipta Maiti is a professor of chemical sciences at the Tata Institute of Fundamental Research (Mumbai, India). His research focuses on protein aggregation, neurotransmission and development of microscopy techniques. He organizes the long-running annual FCS Workshop in India. He is the co-founder and president of the Fluorescence Society (India) and president of the International Society on Optics Within Life Sciences (OWLS, Germany). He is also a fellow of the Indian Academy of Sciences.
Radek Macháň has worked as imaging scientist in microscopy core facilities since 2016, first at Charles University in Prague and then at the Singapore Centre for Environmental Life Sciences Engineering located at Nanyang Technological University in Singapore. Throughout his work at the microscopy core facilities, Radek has continued to promote FCS and related techniques by assisting facility users with FCS experiments and by teaching FCS at courses and practical workshops.
作者簡介(中文翻譯)
Thorsten Wohland 是新加坡國立大學（NUS）生物科學與化學系的教授。他曾獲得亞歷山大·馮·洪堡研究獎學金及新加坡國立大學科學院的傑出科學家獎。他的研究興趣在於螢光技術的發展及其在生物系統中的應用。他持續開發新的螢光相關技術（FCS）模式，並熱衷於教授FCS，這激勵了本書的撰寫。
Sudipta Maiti 是印度孟買塔塔基礎研究所的化學科學教授。他的研究專注於蛋白質聚集、神經傳遞及顯微技術的發展。他在印度組織了長期舉辦的年度FCS研討會。他是印度螢光學會的共同創辦人及會長，也是德國生命科學光學國際學會（OWLS）的會長。他同時也是印度科學院的院士。
Radek Macháň 自2016年以來一直在顯微鏡核心設施擔任影像科學家，最初在布拉格的查理大學，然後在新加坡南洋理工大學的環境生命科學工程中心工作。在顯微鏡核心設施的工作中，Radek 持續推廣FCS及相關技術，通過協助設施使用者進行FCS實驗以及在課程和實作研討會中教授FCS。
