Total Internal Reflection Flurescence: Total Internal Reflection Excitation and Near Field Emissionoptical Evanescence Microscopy (Tirk)

Axelrod, Daniel

  • 出版商: IOP Publishing Ltd
  • 出版日期: 2022-09-13
  • 售價: $6,400
  • 貴賓價: 9.5$6,080
  • 語言: 英文
  • 頁數: 200
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 0750333499
  • ISBN-13: 9780750333498
  • 海外代購書籍(需單獨結帳)

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商品描述

This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes.


Key Features:


  • Suitable for both basic and advanced levels
  • Offers combinations with related techniques
  • Relationship of evanescent effects between excitation and emission explained
  • Practical guide to the optics as well