Membrane Proteins in Aqueous Solutions: From Detergents to Amphipols
暫譯: 水相中的膜蛋白：從清潔劑到兩性聚合物
Popot, Jean-Luc
- 出版商: Springer
- 出版日期: 2019-02-01
- 售價: $7,350
- 貴賓價: 9.5 折 $6,983
- 語言: 英文
- 頁數: 708
- 裝訂: Quality Paper - also called trade paper
- ISBN: 3030103242
- ISBN-13: 9783030103248
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商品描述
This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications.
Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users.
Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.
商品描述(中文翻譯)
這本書是首部專門探討如何在非自然環境中處理膜蛋白的挑戰性藝術的著作，這一過程在生物學和藥物研究中至關重要，但卻充滿了困難和陷阱。由該領域的頂尖專家之一撰寫的《水相中的膜蛋白》對於任何膜生物學實驗室的成員來說都是易於理解的。在介紹膜蛋白的結構、功能、動力學、合成、自然環境及脂質相互作用後，作者討論了使用清潔劑提取膜蛋白的原則、清潔劑引起的不穩定化機制、對策，以及在開發具有較弱變性特性的清潔劑方面的最新進展。書中描述了清潔劑的非傳統替代品，包括雙層小球、納米圓盤、兩親肽、氟化表面活性劑和兩親聚合物，並比較了它們的相對優缺點。各類兩親聚合物的合成及其溶液性質也被介紹，還有膜蛋白/兩親聚合物複合物的形成及性質，以及將兩親聚合物捕獲的蛋白質轉移到清潔劑、納米圓盤、脂質介相或活細胞中的過程。本書的最後幾章涉及應用：膜蛋白的體外折疊和無細胞表達、溶液研究、核磁共振（NMR）、結晶學、電子顯微鏡、質譜、兩親聚合物介導的膜蛋白固定化，以及生物醫學應用。
本書的重要特點包括介紹各種生物物理技術的基礎和最新進展的入門部分，以及組織廣泛文獻的主題表格。書中各處的框框和附錄解釋了技術方面的內容，並由經驗豐富的用戶提供和評論了十二個詳細的實驗方案，涵蓋從膜蛋白的體外折疊到單顆粒電子冷凍顯微鏡的各個方面。
《水相中的膜蛋白》提供了膜蛋白生物化學和生物物理學的簡明易懂的介紹，以及對傳統和非傳統表面活性劑的性質和用途的全面覆蓋。它將對基礎和應用研究實驗室以及作為學生、講師、研究人員和專業人士的教學輔助工具非常有用。
作者簡介
Jean-Luc Popot, born 1948, studied biology, chemistry, and biophysics in Orléans and Orsay. In 1971, he took a permanent position with the Collège de France. In J.-P. Changeux's laboratory, at the Pasteur Institute, he applied electrophysiology and biophysical and biochemical approaches to functional and structural studies of the nicotinic acetyl-cho-li-ne receptor. In 1982, he joined D.M. Engelman at Yale University as a visiting scientist. His work at Yale, which bore princi-pal-ly on refolding bacterio-rhod-opsin from dena-tu-red fragments and studying the refolded structure crystallographically, led the two of them to propose, in 1990, an influential model for the folding of α-helical membrane proteins. In 1985, he joined the laboratory of P. Joliot at the Institut de Biologie Physico-Chimique (Paris). His group car-ried out neutron diffraction and model building work on bacterio-rhod-opsin and bio-che-mical studies on Photo-sys-tem II and cytochrome b6 f. In 1996, he became Research Director at the Centre National de la Recher--che Scienti-fi-que and created his own laboratory, where the X-ray struc-tu-re of the b6 f was solved in 2003. In parallel, he pursued the development of sequence analysis approa-ches and, in collabo-ra-tion with chemists and physical chemists, designed and validated non-conventional surfact-ants aimed at facilitating membrane protein solu-tion studies, most notably amphi--pathic polymers ('amphipols') and fluorinated surfactants. He retired in 2013 and, along with his wife, splits his time between restoring an old house in Languedoc, reading, writing, hiking, photographing, cooking, and enjoying the company of kith and kin.
作者簡介(中文翻譯)
讓-盧克·波波（Jean-Luc Popot），1948年出生，在奧爾良和奧爾塞學習生物學、化學和生物物理學。1971年，他在法國高等學院（Collège de France）獲得永久職位。在J.-P. Changeux的實驗室中，他在巴斯德研究所應用電生理學以及生物物理和生化方法進行尼古丁乙醯膽鹼受體的功能和結構研究。1982年，他作為訪問科學家加入耶魯大學的D.M. Engelman實驗室。他在耶魯的工作主要集中在從變性片段重新折疊細菌視紫紅質（bacteriorhodopsin）並對重新折疊的結構進行晶體學研究，這使他們在1990年提出了一個有影響力的模型，解釋α-螺旋膜蛋白的折疊過程。1985年，他加入巴黎物理化學生物學研究所（Institut de Biologie Physico-Chimique）P. Joliot的實驗室。他的團隊對細菌視紫紅質進行了中子衍射和模型構建工作，並對光合系統II和細胞色素b6f進行了生化研究。1996年，他成為法國國家科學研究中心（Centre National de la Recherche Scientifique）的研究主任，並創建了自己的實驗室，該實驗室於2003年解決了b6f的X射線結構。與此同時，他推進了序列分析方法的發展，並與化學家和物理化學家合作，設計和驗證了旨在促進膜蛋白溶解研究的非常規表面活性劑，最著名的是兩親性聚合物（"amphipols"）和氟化表面活性劑。他於2013年退休，與妻子一起在朗格多克（Languedoc）修復一棟老房子，並分配時間閱讀、寫作、健行、攝影、烹飪，享受與親友的相聚。
