Intracellular Lipid Transport: Methods and Protocols (細胞內脂質運輸:方法與實驗步驟)

Drin, Guillaume

  • 出版商: Humana Press
  • 出版日期: 2025-02-14
  • 售價: $9,640
  • 貴賓價: 9.5$9,158
  • 語言: 英文
  • 頁數: 357
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 1071643177
  • ISBN-13: 9781071643174
  • 尚未上市,無法訂購

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商品描述

This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the book continues with in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoint, how lipid transfer proteins function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Intracellular Lipid Transport: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.

商品描述(中文翻譯)

這本全面更新的書籍探討了不同類別的脂質運輸蛋白,例如脂質轉移蛋白、翻轉酶和隨機酶,以及在解決它們的生物功能方面的重要進展。書籍的前半部分介紹了測量脂質在真核細胞(包括植物細胞)內部或原核細胞的細胞器膜之間或膜內部移動的方法,接下來則以體外或計算模擬的方法,從生化和結構的角度更深入地定義脂質轉移蛋白的功能。本書是為了成功的《Methods in Molecular Biology》系列而撰寫,章節包括各自主題的介紹、所需材料和試劑的清單、逐步且易於重現的實驗室操作流程,以及故障排除和避免已知陷阱的建議。

權威且最新的《Intracellular Lipid Transport: Methods and Protocols, Second Edition》是研究人員尋求揭示這一關鍵且常常難以捉摸的細胞過程各個方面的理想指南。