Photosynthesis Research Protocols

1. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher Plants Yasusi Yamamoto, Jing Leng, and Jian-Ren Shen 2. Isolation of Photosystem I Submembrane Fractions Johanne Harnois, Najoua Msilini, and Robert Carpentier 3. Isolation of Photosystem II Reaction Center Complexes from Plants Michael Seibert and Rafael Picorel 4. Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803 Dmitrii V. Vavilin 5. Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria Jian-Ren Shen, Keisuke Kawakami, and Hiroyuki Koike 6. Isolation of Cytochrome b6f Complex from Grana and Stroma Membranes from Spinach Chloroplasts Elżbieta Romanowska 7. Purification and Crystallization of the Cytochrome b6f Complex Danas Baniulis, Huamin Zhang, Taisaya Zakharova, S. Saif Hasan, and William A. Cramer 8. Purification of Plastocyanin and Cytochrome c6 from Plants, Green Algae, and Cyanobacteria José A. Navarro, Manuel Hervás, and Miguel A. De la Rosa 9. Isolation and Identification of Chloroplast Lipids Norihiro Sato and Mikio Tsuzuki 10. Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants Rafael Picorel, Miguel Alfonso, and Michael Seibert 11. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex Wolfgang Rühle and Harald Paulsen 12. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies with Light-Inducible Structural Transitions Ilian Simidjiev, Zsuzsanna Várkonyi, Petar H. Lambrev, and Győző Garab 13. Protein Targeting across and into Chloroplast Membranes Steven M. Theg and Shari M. Lo 14. Proteomic Analysis of Thylakoid Membranes Venkateswarlu Yadavalli, Sreedhar Nellaepalli, and Rajagopal Subramanyam 15. Thylakoid Phosphoproteins: Identification of Phosphorylation Sites Anne Rokka, Eva-Mari Aro, and Alexander V. Vener 16. Direct Detection of Free Radicals and Reactive Oxygen Species in Thylakoids Éva Hideg, Tamás Kálai, and Kálmán Hideg 17. Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher Plants Nobuyoshi Nijo, Björn Lundin, Miho Yoshioka, Noriko Morita, and Yasusi Yamamoto 18. Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure-Function Characterization David Joly, Sridharan Govindachary, and Mário Fragata 19. Physical and Chemical Immobilization Methods of Photosynthetic Materials Lise Barthelmebs, Robert Carpentier, and Régis Rouillon 20. Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana Verónica Albrecht, Gonzalo M. Estavillo, Abby J. Cuttriss, and Barry J. Pogson 21. Expression of Genes in Cyanobacteria: Adaptation of Endogenous Plasmids as Platforms for High-Level Gene Expression in Synechococcus sp. PCC 7002 Yu Xu, Richard M. Alvey, Patrick O. Byrne, Joel E. Graham, Gaozhong Shen, and Donald A. Bryant 22. Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803 Julian J. Eaton-Rye 23. A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii Vellupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber 24. Rapid Isolation of Intact Chloroplasts from Spinach Leaves David Joly and Robert Carpentier 25. Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses Elżbieta Romanowska and Eugeniusz Parys 26. Isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Leaves A. Elizabete Carmo-Silva, Csengele Barta, and Michael E. Salvucci 27. Quantifying the Amount and Activity of Rubisco in Leaves David S. Kubien, Christopher M. Brown, and Heather J. Kane 28. Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli Csengele Barta, A. Elizabete Carmo-Silva, and Michael