Metabolic Profiling: Methods and Protocols
暫譯: 代謝剖析:方法與實驗流程
Deda, Olga, Gika, Helen G., Wilson, Ian D.
- 出版商: Humana Press
- 出版日期: 2025-03-10
- 售價: $9,720
- 貴賓價: 9.5 折 $9,234
- 語言: 英文
- 頁數: 309
- 裝訂: Hardcover - also called cloth, retail trade, or trade
- ISBN: 1071643339
- ISBN-13: 9781071643334
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This second edition volume expands on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS) and the targeted determination of metanephrines in urine via LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and comprehensive, Metabolic Profiling: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in incorporating best practices in metabolic phenotyping and learning more about this rapidly advancing field.商品描述(中文翻譯)
這本第二版的書籍在前一版的基礎上擴展了新章節,並更新了有關代謝表型發展和實踐的最新進展的討論。本書的章節涵蓋了無目標代謝表型中的質量控制;質量保證過程的實施;仔細的生物和化學信息數據分析;使用反相和離子對液相色譜質譜(LC-MS);利用超臨界流體色譜(SFC-MS)進行定量脂質分析,以及通過液相色譜串聯質譜(LC-MS/MS)針對性地測定尿液中的美托腎上腺素。這本書採用了成功的《分子生物學方法(Methods in Molecular Biology)》系列格式,章節包括各自主題的介紹、所需材料和試劑的清單、逐步的、易於重現的實驗室操作流程,以及故障排除和避免已知陷阱的提示。
全面而詳盡的《代謝剖析:方法與協議,第二版》是所有有興趣將最佳實踐納入代謝表型並進一步了解這一快速發展領域的研究人員的寶貴資源。