Base Excision Repair Pathway: Methods and Protocols
Bhakat, Kishor K., Hazra, Tapas K.
- 出版商: Humana Press
- 出版日期: 2024-08-15
- 售價: $6,190
- 貴賓價: 9.5 折 $5,881
- 語言: 英文
- 頁數: 262
- 裝訂: Quality Paper - also called trade paper
- ISBN: 1071633759
- ISBN-13: 9781071633755
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商品描述
This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topis, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.
Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.
商品描述(中文翻譯)
這本詳細的專著提供了一套全面的實驗協議和有用的策略,以檢視透過基本切除修復(Base Excision Repair, BER)途徑修復受損碱基的過程,無論是在體外(in vitro)還是在細胞中。書中首先介紹了多種分子和細胞技術,以檢查從雙鏈DNA或包裹在核小體中的DNA中切除受損碱基的過程,接著涵蓋了檢測和定量受損碱基、蛋白質-DNA交聯和雙鏈斷裂的程序,實驗程序以傳統的串聯親和純化法識別DNA修復蛋白的相互作用體,並隨後進行質譜分析,以及分析DNA修復蛋白的全基因組結合和腫瘤中DNA損傷反應基因的拷貝數變異。這本書是為了成功的《Methods in Molecular Biology》系列而撰寫,章節包括各自主題的介紹、所需材料和試劑的清單、逐步且易於重現的實驗室協議,以及故障排除和避免已知陷阱的提示。《Base Excision Repair Pathway: Methods and Protocols》權威且實用,為初學者和專家提供了寶貴的資源,以檢視透過BER途徑中不同的蛋白質修復各類型DNA損傷的過程,無論是在體外還是在細胞中。