Myofibroblasts: Methods and Protocols

1. 50 Years of Myofibroblasts: How the Myofibroblast Concept Evolved

Giulio Gabbiani

Section I: Fundamental Methods to Study Myofibroblast Biology

2. Myofibroblast Functions in Tissue Repair and Fibrosis: An Introduction

Victor J. Thannickal

3. Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology

Fereshteh Younesi, Dong Ok Son, Joao Firmino, and Boris Hinz

4. Fibroblast and Myofibroblast Subtypes: Single Cell Sequencing

Hesham Soliman, Lin Wei Tung, and Fabio M.V. Rossi

5. Myofibroblast Adhesome Analysis by Mass Spectrometry

Christopher A. McCulloch

6. Myofibroblast TGF-β Activation Measurement In Vitro

Joanne Porte, Gisli Jenkins, and Amanda L. Tatler

7. Contraction Measurements Using Three-Dimensional Fibrillar Collagen Gel Lattices

James Griffith and William L. Berry

8. Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro

Tamara Rosell-García and Fernando Rodriguez-Pascual

9. Methods for Studying Myofibroblast Apoptotic Pathways

Yan Zhou and David Lagares

10. Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices

Tamara Cruz, Ana L. Mora, and Mauricio Rojas

11. The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing

Simon Stebler and Michael Raghunath

Section II: Myofibroblast Mechanobiology

12. Why Stress Matters: An Introduction

Daniel J. Tschumperlin

13. Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation

Natalie M. Landry, Sunil G. Rattan, and Ian M.C. Dixon

14. Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy

Shuying Yang, Fernando R. Valencia, Benedikt Sabass, and Sergey V. Plotnikov

15. Nucleocytoplasmic Shuttling of the Mechanosensitive Transcription Factors MRTF and YAP/TAZ

Michael Kofler and András Kapus

16. Atomic Force Microscopy for Live-Cell and Hydrogel Measurement

Alexander Whitehead, Natalie J. Kirkland, and Adam J. Engler

17. Method for Investigating Fibroblast Durotaxis

Hossam Kadry, David Lagares, and Taslim A. Al-Hilal

18. Decellularized Extracellular Matrix (ECM) as a Model to Study Fibrotic ECM Mecha