The Mitoribosome: Methods and Protocols

Barrientos, Antoni, Fontanesi, Flavia

The Mitoribosome: Methods and Protocols
  • 出版商: Humana Press
  • 出版日期: 2024-05-25
  • 售價: $6,560
  • 貴賓價: 9.5 折 $6,232
  • 語言: 英文
  • 頁數: 346
  • 裝訂: Quality Paper - also called trade paper
  • ISBN: 107163173X
  • ISBN-13: 9781071631737

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商品描述

This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to study mitochondrial mRNAs that are translated on mitoribosomes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, The Mitoribosome: Methods and Protocols, aims to be a comprehensive guide for researchers in the field.

商品描述(中文翻譯)

本卷提供了經典與新穎的方法，以研究不同物種中粒線體核糖體的結構、組裝途徑及蛋白質合成能力。在介紹該主題的基本概念後，各方法章節呈現基於冷凍電子斷層掃描、冷凍電子顯微鏡技術、粒線體核糖體純化技術、粒線體翻譯測定及研究在粒線體核糖體上翻譯的粒線體mRNA的方法的詳細協議。該書採用成功的《Methods in Molecular Biology》系列格式編寫，每章包括主題介紹、所需材料和方法清單、故障排除提示及已知陷阱，並提供逐步、易於重現的協議。

權威且前沿的《The Mitoribosome: Methods and Protocols》旨在成為該領域研究人員的綜合指南。

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