Cancer Cell Culture: Methods and Protocols
Movia, Dania, Prina-Mello, Adriele
- 出版商: Humana Press
- 出版日期: 2024-05-19
- 售價: $6,560
- 貴賓價: 9.5 折 $6,232
- 語言: 英文
- 頁數: 290
- 裝訂: Quality Paper - also called trade paper
- ISBN: 107163058X
- ISBN-13: 9781071630587
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This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two contains technical chapters that illustrate step-by-step methodologies for specific cancer cell culture methods. The methods discussed range from the generation of isogenic cancer cell lines, the use of serum-free growth conditions, and three-dimensional cell cultures and their specific assays for the efficacy assessment of new anticancer therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Cancer Cell Culture: Methods and Protocols is a valuable tool to help researchers involved in this important field to further improve or advance their models for cancer research.商品描述(中文翻譯)
本書探討了在臨床前癌症研究中使用的最新細胞模型集合,包括二維和三維培養技術。本書的章節分為兩部分。第一部分討論了二維癌細胞培養、空氣液體界面上的細胞模型,以及三維複雜球體模型和專門的疾病動物模型的最新進展。第二部分包含了具體癌細胞培養方法的技術章節。所討論的方法範圍包括同源癌細胞株的生成、無血清生長條件的使用,以及三維細胞培養及其特定的新抗癌療法效力評估方法。本書以廣受好評的《分子生物學方法》系列的格式撰寫,章節包括對各個主題的介紹、所需材料和試劑的列表、逐步可重複的實驗室協議,以及疑難解答和避免已知問題的提示。《癌細胞培養:方法與協議》是一本尖端且全面的書籍,對於在這一重要領域從事研究的人員來說,是一個有價值的工具,可以進一步改進或推進他們的癌症研究模型。