A Practical Guide to Optical Microscopy
暫譯: 光學顯微鏡實用指南

Girkin, John

A Practical Guide to Optical Microscopy

商品描述

Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available.

 

This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist.

It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available.

Features:

  • The first book to cover all current optical microscopy methods for practical applications
  • Written to be understood by a non-optical expert with inserts to provide the physical science background
  • Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book

商品描述(中文翻譯)

光學顯微鏡被廣泛應用於材料工程、體內觀察和臨床診斷等多個領域，並且得益於最新的技術進步，現有的方法數量迅速增長。

本書旨在為用戶提供實用指南，幫助他們選擇並使用最適合其應用的方法。它探討了不同形式的光學顯微鏡背後的原理，無需使用複雜的數學，從而幫助讀者理解如何利用特定方法並解釋結果。詳細的物理學內容以框選的形式提供，非專業人士可以選擇跳過。

這是生命科學和物理科學研究小組及實驗室中不可或缺的工具，作為提供實用信息的首要來源，指導經驗較少的用戶（或對特定方法論不熟悉的人）了解可用的技術範圍。

**特色：**

- 首本涵蓋所有當前光學顯微鏡方法以供實際應用的書籍
- 以非光學專家的理解為目標，並插入提供物理科學背景的內容
- 將傳統的廣域和共聚焦顯微鏡與先進的非線性和超解析方法整合於一本書中

作者簡介

John Girkin is professor of Biophysics at Durham University and Director of the Biophysical Sciences Institute in Durham. He moved to Durham in 2009 to take up this role having previously founded the Centre for Biophotonics at Strathclyde University, Glasgow where he was one of the first leaders at the Institute of Photonics. Originally trained as a physicist at Oxford and with a PhD from Southampton University (in Laser Spectroscopy of Atomic Hydrogen) he worked for ten years in industry including developing the world's first diode laser retinal photocoagulator and diode pumped Nd: Yag laser. He moved back into academia in 1996 as one of the original Research Team Leaders at the Institute of Photonics, Strathclyde University.

His research focuses on the development of novel optical instrumentation to help solve challenges within the life sciences. His research covers a very broad range of activities from dental imaging in the near infrared through to rapid-genomic screening but an ongoing theme has been in developing and applying optical microscopy methods to life science challenges. His initial focus was in non-linear microscopy and he was one of the first pioneers in the use of adaptive optics in microscopy and has more recently been developing advanced forms of single plane illumination microscope for in vivo zebra fish imaging. A constant within his research has been to select the most suitable method for a specific task.

He has published over 100 peer-reviewed publications, is a Fellow of both the Institute of Physics and the Optical Society of America serving on both national and international review panels in the area of biophotonics and microscopy.

作者簡介(中文翻譯)

約翰·吉爾金（John Girkin）是達勒姆大學的生物物理學教授及生物物理科學研究所所長。他於2009年移居達勒姆，擔任此職位，之前曾在格拉斯哥的斯特拉斯克萊德大學創立生物光子學中心，並且是光子學研究所的首批領導者之一。他最初在牛津大學接受物理學訓練，並在南安普敦大學獲得博士學位（研究主題為原子氫的激光光譜學），在業界工作了十年，包括開發世界上第一個二極體激光視網膜光凝固儀和二極體泵浦的Nd:YAG激光。1996年，他回到學術界，成為斯特拉斯克萊德大學光子學研究所的首批研究團隊領導者之一。

他的研究專注於開發新型光學儀器，以幫助解決生命科學中的挑戰。他的研究涵蓋了從近紅外牙科成像到快速基因組篩查的廣泛活動，但持續的主題是開發和應用光學顯微鏡方法來解決生命科學的挑戰。他最初的重點是非線性顯微鏡，並且是自適應光學在顯微鏡中應用的首批先驅之一，最近則在開發用於in vivo斑馬魚成像的先進單平面照明顯微鏡。他的研究中始終如一的是選擇最適合特定任務的方法。

他已發表超過100篇經過同行評審的出版物，是物理學會和美國光學學會的會士，並在生物光子學和顯微鏡領域的國內外評審小組中任職。

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