Cilia: Methods and Protocols
暫譯: 纖毛:方法與實驗步驟
Mennella, Vito
- 出版商: Humana Press
- 出版日期: 2024-10-20
- 售價: $6,340
- 貴賓價: 9.5 折 $6,023
- 語言: 英文
- 頁數: 278
- 裝訂: Quality Paper - also called trade paper
- ISBN: 1071635093
- ISBN-13: 9781071635094
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This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.商品描述(中文翻譯)
本卷涵蓋了纖毛複雜性研究的最新進展。所述的實驗流程包括不同纖毛組織的基因組學、蛋白質組學、成像和功能分析,以及它們在纖毛生物學中的廣泛應用。本書的章節主要集中於研究多纖毛細胞的方法,並討論了如 SARS-CoV-2 感染人類原代鼻多纖毛上皮細胞;纖毛蛋白的擴展顯微鏡技術;小鼠大腦多纖毛細胞中的中心粒增幅的活體成像;纖毛運動的生物物理特性;以及黏液纖毛運輸裝置的構建等主題。這本書採用成功的《Methods in Molecular Biology》系列格式編寫,章節包括各自主題的介紹、所需材料和試劑的清單、逐步的可重複實驗室流程,以及故障排除和避免已知陷阱的提示。
前沿且全面的《Cilia: Methods and Protocols》是對於希望深入了解這一發展領域的研究人員來說,極具價值的資源。