Myeloid-Derived Suppressor Cells: Volume 184

Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive and detailed protocols to assess the critical role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume presents twelve interesting protocols that address the isolation of MDSCs in murine models, the detection of mouse and human MDSCs by flow cytometry, T-cell proliferation assays, isolation of MDSC-derived exosomes and their immunosuppressive function, characterization of lysosomal lipase in MDSCs, detection of MDSC-derived nitric oxide, and other in vivo and ex vivo assays.